Selection of Immature Bovine Oocytes Using Brilliant Cresyl Blue Enhances Nuclear Maturity after Vitrification
نویسندگان
چکیده
منابع مشابه
Selection of Ovine Oocytes by Brilliant Cresyl Blue Staining
Sheep oocytes derived from the ovaries collected from the slaughterhouse are often used for research on in vitro embryo production, animal cloning, transgenesis, embryonic stem cells, and other embryo biotechnology aspects. Improving the in vitro culture efficiency of oocytes can provide more materials for similar studies. Generally, determination of oocyte quality is mostly based on the layers...
متن کاملDevelopmental competence of bovine oocytes selected based on follicle size and using the brilliant cresyl blue (BCB) test
BACKGROUND Many studies reported that follicle size has an essential role in developmental potential of oocytes. Also, the brilliant cresyl blue (BCB) test is one of the most important criteria in selection of more competent oocytes. OBJECTIVE Selection of developmentally competent bovine oocytes. MATERIALS AND METHODS A total of 1730 bovine cumulus oocyte complexes (COCs) were recovered fr...
متن کاملBrilliant Cresyl Blue in Sap of Nitella
It has been shown by several investigators I that when the pl-I value of the external dye solutions is increased, the rate of accumulation of a basic dye in the cell sap is accelerated. Two different explanations for this have been given. Some have accounted for it on the basis that the basic dye enters the cell in the form of a dye hydrate and combines with the substances in the cell; and that...
متن کاملDevelopmental competence of prepubertal goat oocytes selected with brilliant cresyl blue and matured with cysteamine supplementation.
The aim of this study was to assess the effect of oocyte selection using the brilliant cresyl blue (BCB) test plus the addition of cysteamine to the in vitro maturation (IVM) medium to improve the in vitro embryo development of prepubertal goat oocytes. The oocytes were exposed to 26 microM BCB and classified according to their cytoplasm coloration: BCB+ (oocytes with blue cytoplasm) and BCB- (...
متن کاملVitrification of immature bovine oocytes using glass micropipettes under normal or super-cooled LN Vitrification and thawing: Two vitrification solutions
Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro. Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in medium TCM-199 with 10% FBS. Immediately, within a minute they are plunged into liquid nitrogen us...
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ژورنال
عنوان ژورنال: Journal of Animal and Veterinary Advances
سال: 2010
ISSN: 1680-5593
DOI: 10.3923/javaa.2010.2710.2713